Activation Test

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The activation test is a diagnostic performed the first time an Evangelion unit is activated. An activation test is not necessary for future activations.

Procedure

The Evangelion is hooked up to an umbilical cable, and its internal batteries are charged. After this process is complete, the signal plug is ejected and the entry plug is inserted into the body of the Evangelion. As opposed to a standard activation, the optic nerves are not activated. Power is supplied to the Evangelion unit until it reaches an internal threshold, at which point the Evangelion is deemed to be activated and fit for future re-activations.

Evangelion Unit-00

In the case of Eva-00, its initial activation was unsuccessful. After going berserk, it was cut off from power and allowed to exhaust its internal batteries. After it lost power, a signal plug was inserted, effectively rendering the unit useless. The unit's second activation went without problems.

Evangelion Unit-01

It is not specified when Eva-01 underwent its first activation, but it is a possibility that the unit never had a proper activation test and was sent directly into combat against Sachiel with Shinji Ikari as its pilot. It is also possible that Rei Ayanami may have been the pilot used for Eva-01's activation test, but the pilot-swap test seen in Episode 14 suggests this was not the case.

Evangelion Unit-02

Asuka activated and trained with her Evangelion in Germany, long before it ever made an appearance.

Evangelion Unit-03

Eva-03 was infected by Bardiel, which entered the Eva during transit and lay dormant until its activation test. During the activation test the Angel infected the Eva, forcing Nerv to destroy it.

Evangelion Unit-04

Eva-04's activation test is shrouded in mystery. The only known fact is that its S² engine was activated, before enveloping Nerv's Nevada branch in a Dirac Sea. Since no pilot is ever mentioned in connection with the Eva and the test was specifically an engine test, it would probably have undergone activation testing later.

Mass Production Evangelions

All the MPEs were likely activated at Seele before appearing in End of Evangelion. Because they are clearly piloted by dummy plugs, it stands to reason that they must have been activated with the plugs as well.

In Rebuild

Evangelion Provisional Unit-05

It is assumed that Eva-05 finished its original activation test seconds before it appeared on-screen. The text characters covering the walls of her entry plug suggest that some sort of internal diagnostic occurred following its first (and only) activation.

Evangelion Mark.06

Nothing is known about the Mk.06's activation or if it even needed an activation test. Since this Eva was built in utmost secrecy on the moon and sent down to earth in a hurry following Zeruel's attack, it probably never went any sort of testing.

Evangelion Unit-08

Eva-08 was presumably tested sometime during the 14 year timeskip.

Evangelion Mark.09

Nothing is known about the Mark.09, or whether it underwent any sort of activation test.

Evangelion 13

No testing seen onscreen, apparently sent directly into operation.