Kaji looking at Unit One, which is looking up at the heavens and howling.
RITSUKO:“(OFF) stop the Eva anymore.”
UrsusArctos: There are some very subtle throwbacks in this scene. The lampposts between Kaji and the Eva have tops in the shape of an inverted pyramid and their general shape is similar to the Tower of Babel from Nadia. The Tower of Babel was a communications device meant to reach to other worlds, repurposed as a weapon. The Evangelions are derived from lifeforms meant to communicate with other worlds, repurposed as weapons. The very fact that Yui is looking up to the heavens (through all the rock above) and howling hints at her ultimate goal.
Mbryo: Eva 01's roar here is absolutely horrific, not quite a bestial roar but not quite human as well. It's Megumi Hayashibara, Yui and Rei's seiyuu herself screaming, only the sound is pitched-down to achieve a guttural effect.