Ikari ascertains the situation. He faces the monitor and speaks.
Ikari:“Fuyutsuki.”
TheAtomicWaffle: Gendo's expression looks rather sad in this shot, strange for a man like him.
OMF: I always took Gendo's expression here to be one of regret and, dare I say it, remorse. He is, after all, the primary reason Shinji is such a wreck.
Reichu: To me, this looks more like an expression of, “You disappoint me, Boy.” :shrug:
Sailor Star Dust: Given what we discover in EoE, Gendo's expression (it's in the eyes) strikes me as remorseful too. Could be a mix of disappointment and remorse, of course. No need for either/or.